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anti prss1 sheep polyclonal antibody  (R&D Systems)


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    R&D Systems anti prss1 sheep polyclonal antibody
    A , Secreted trypsinogen protein (arrow) in the conditioned medium 24 hours and 48 hours after transfection was assessed by SDS-PAGE and Coomassie Blue staining. Representative gels from 3 independent transfections are shown. B , Trypsinogen levels in the conditioned medium were determined by trypsin activity measurement after activation with enteropeptidase. Individual values from 4 transfections with duplicates (n = 8) are shown, with the mean and SD indicated. C , Western blot analysis of trypsinogen (arrow) levels in cell lysates 48 hours post-transfection. Alpha-tubulin was measured as loading control. Representative blots are shown. D , <t>PRSS1</t> mRNA levels were measured 48 hours after transfection by reverse-transcription quantitative PCR and expressed as fold change relative to the average value of the cDNA construct. Individual values from 3 transfections with duplicates (n = 6) are shown, with the mean and SD indicated. E , Splicing of PRSS1 mRNA expressed from cDNA and minigene constructs was analyzed 48 hours after transfection by reverse-transcription PCR and agarose gel electrophoresis. The arrow indicates the correctly spliced PRSS1 band. The smaller faint band in the minigene samples indicated by the asterisk corresponds to an aberrant splice product in which nucleotide c.40 was spliced to c.114 resulting in the deletion of the mini-intron and an extra 74 nucleotides from exon 2.
    Anti Prss1 Sheep Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+prss1+sheep+polyclonal+antibody/Human+Trypsin+1%2FPRSS1+Antibody/pmc12970870-41-1-8
    Average 90 stars, based on 9 article reviews
    anti prss1 sheep polyclonal antibody - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Minigenes for heterologous expression of human and mouse cationic trypsinogen"

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen

    Journal: PLOS One

    doi: 10.1371/journal.pone.0343840

    A , Secreted trypsinogen protein (arrow) in the conditioned medium 24 hours and 48 hours after transfection was assessed by SDS-PAGE and Coomassie Blue staining. Representative gels from 3 independent transfections are shown. B , Trypsinogen levels in the conditioned medium were determined by trypsin activity measurement after activation with enteropeptidase. Individual values from 4 transfections with duplicates (n = 8) are shown, with the mean and SD indicated. C , Western blot analysis of trypsinogen (arrow) levels in cell lysates 48 hours post-transfection. Alpha-tubulin was measured as loading control. Representative blots are shown. D , PRSS1 mRNA levels were measured 48 hours after transfection by reverse-transcription quantitative PCR and expressed as fold change relative to the average value of the cDNA construct. Individual values from 3 transfections with duplicates (n = 6) are shown, with the mean and SD indicated. E , Splicing of PRSS1 mRNA expressed from cDNA and minigene constructs was analyzed 48 hours after transfection by reverse-transcription PCR and agarose gel electrophoresis. The arrow indicates the correctly spliced PRSS1 band. The smaller faint band in the minigene samples indicated by the asterisk corresponds to an aberrant splice product in which nucleotide c.40 was spliced to c.114 resulting in the deletion of the mini-intron and an extra 74 nucleotides from exon 2.
    Figure Legend Snippet: A , Secreted trypsinogen protein (arrow) in the conditioned medium 24 hours and 48 hours after transfection was assessed by SDS-PAGE and Coomassie Blue staining. Representative gels from 3 independent transfections are shown. B , Trypsinogen levels in the conditioned medium were determined by trypsin activity measurement after activation with enteropeptidase. Individual values from 4 transfections with duplicates (n = 8) are shown, with the mean and SD indicated. C , Western blot analysis of trypsinogen (arrow) levels in cell lysates 48 hours post-transfection. Alpha-tubulin was measured as loading control. Representative blots are shown. D , PRSS1 mRNA levels were measured 48 hours after transfection by reverse-transcription quantitative PCR and expressed as fold change relative to the average value of the cDNA construct. Individual values from 3 transfections with duplicates (n = 6) are shown, with the mean and SD indicated. E , Splicing of PRSS1 mRNA expressed from cDNA and minigene constructs was analyzed 48 hours after transfection by reverse-transcription PCR and agarose gel electrophoresis. The arrow indicates the correctly spliced PRSS1 band. The smaller faint band in the minigene samples indicated by the asterisk corresponds to an aberrant splice product in which nucleotide c.40 was spliced to c.114 resulting in the deletion of the mini-intron and an extra 74 nucleotides from exon 2.

    Techniques Used: Transfection, SDS Page, Staining, Activity Assay, Activation Assay, Western Blot, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Construct, Agarose Gel Electrophoresis

    Related Articles

    Transfection:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    SDS Page:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    Staining:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    Activity Assay:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    Activation Assay:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    Western Blot:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    Control:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    Reverse Transcription:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    Real-time Polymerase Chain Reaction:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    Construct:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.

    Agarose Gel Electrophoresis:

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen
    Article Snippet: Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).Cell lysates (10 μL per lane) were electrophoresed on 15% mini-gels and transferred to Immobilon-P membrane (catalog number IPVH00010, Sigma).. The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.. As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.As secondary antibody, horseradish peroxidase (HRP)-conjugated donkey polyclonal anti-sheep IgG (catalog number HAF016, R&D Systems) was used at 1:2000 dilution.



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    anti prss1 sheep polyclonal antibody  (R&D Systems)
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    R&D Systems anti prss1 sheep polyclonal antibody
    A , Secreted trypsinogen protein (arrow) in the conditioned medium 24 hours and 48 hours after transfection was assessed by SDS-PAGE and Coomassie Blue staining. Representative gels from 3 independent transfections are shown. B , Trypsinogen levels in the conditioned medium were determined by trypsin activity measurement after activation with enteropeptidase. Individual values from 4 transfections with duplicates (n = 8) are shown, with the mean and SD indicated. C , Western blot analysis of trypsinogen (arrow) levels in cell lysates 48 hours post-transfection. Alpha-tubulin was measured as loading control. Representative blots are shown. D , <t>PRSS1</t> mRNA levels were measured 48 hours after transfection by reverse-transcription quantitative PCR and expressed as fold change relative to the average value of the cDNA construct. Individual values from 3 transfections with duplicates (n = 6) are shown, with the mean and SD indicated. E , Splicing of PRSS1 mRNA expressed from cDNA and minigene constructs was analyzed 48 hours after transfection by reverse-transcription PCR and agarose gel electrophoresis. The arrow indicates the correctly spliced PRSS1 band. The smaller faint band in the minigene samples indicated by the asterisk corresponds to an aberrant splice product in which nucleotide c.40 was spliced to c.114 resulting in the deletion of the mini-intron and an extra 74 nucleotides from exon 2.
    Anti Prss1 Sheep Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+prss1+sheep+polyclonal+antibody/Human+Trypsin+1%2FPRSS1+Antibody/pmc12970870-41-1-8
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    anti prss1 sheep polyclonal antibody - by Bioz Stars, 2026-10
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    sheep polyclonal antibody against human cationic trypsinogen  (R&D Systems)
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    A , Secreted trypsinogen protein (arrow) in the conditioned medium 24 hours and 48 hours after transfection was assessed by SDS-PAGE and Coomassie Blue staining. Representative gels from 3 independent transfections are shown. B , Trypsinogen levels in the conditioned medium were determined by trypsin activity measurement after activation with enteropeptidase. Individual values from 4 transfections with duplicates (n = 8) are shown, with the mean and SD indicated. C , Western blot analysis of trypsinogen (arrow) levels in cell lysates 48 hours post-transfection. Alpha-tubulin was measured as loading control. Representative blots are shown. D , <t>PRSS1</t> mRNA levels were measured 48 hours after transfection by reverse-transcription quantitative PCR and expressed as fold change relative to the average value of the cDNA construct. Individual values from 3 transfections with duplicates (n = 6) are shown, with the mean and SD indicated. E , Splicing of PRSS1 mRNA expressed from cDNA and minigene constructs was analyzed 48 hours after transfection by reverse-transcription PCR and agarose gel electrophoresis. The arrow indicates the correctly spliced PRSS1 band. The smaller faint band in the minigene samples indicated by the asterisk corresponds to an aberrant splice product in which nucleotide c.40 was spliced to c.114 resulting in the deletion of the mini-intron and an extra 74 nucleotides from exon 2.
    Sheep Polyclonal Antibody Against Human Cationic Trypsinogen, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A , Secreted trypsinogen protein (arrow) in the conditioned medium 24 hours and 48 hours after transfection was assessed by SDS-PAGE and Coomassie Blue staining. Representative gels from 3 independent transfections are shown. B , Trypsinogen levels in the conditioned medium were determined by trypsin activity measurement after activation with enteropeptidase. Individual values from 4 transfections with duplicates (n = 8) are shown, with the mean and SD indicated. C , Western blot analysis of trypsinogen (arrow) levels in cell lysates 48 hours post-transfection. Alpha-tubulin was measured as loading control. Representative blots are shown. D , PRSS1 mRNA levels were measured 48 hours after transfection by reverse-transcription quantitative PCR and expressed as fold change relative to the average value of the cDNA construct. Individual values from 3 transfections with duplicates (n = 6) are shown, with the mean and SD indicated. E , Splicing of PRSS1 mRNA expressed from cDNA and minigene constructs was analyzed 48 hours after transfection by reverse-transcription PCR and agarose gel electrophoresis. The arrow indicates the correctly spliced PRSS1 band. The smaller faint band in the minigene samples indicated by the asterisk corresponds to an aberrant splice product in which nucleotide c.40 was spliced to c.114 resulting in the deletion of the mini-intron and an extra 74 nucleotides from exon 2.

    Journal: PLOS One

    Article Title: Minigenes for heterologous expression of human and mouse cationic trypsinogen

    doi: 10.1371/journal.pone.0343840

    Figure Lengend Snippet: A , Secreted trypsinogen protein (arrow) in the conditioned medium 24 hours and 48 hours after transfection was assessed by SDS-PAGE and Coomassie Blue staining. Representative gels from 3 independent transfections are shown. B , Trypsinogen levels in the conditioned medium were determined by trypsin activity measurement after activation with enteropeptidase. Individual values from 4 transfections with duplicates (n = 8) are shown, with the mean and SD indicated. C , Western blot analysis of trypsinogen (arrow) levels in cell lysates 48 hours post-transfection. Alpha-tubulin was measured as loading control. Representative blots are shown. D , PRSS1 mRNA levels were measured 48 hours after transfection by reverse-transcription quantitative PCR and expressed as fold change relative to the average value of the cDNA construct. Individual values from 3 transfections with duplicates (n = 6) are shown, with the mean and SD indicated. E , Splicing of PRSS1 mRNA expressed from cDNA and minigene constructs was analyzed 48 hours after transfection by reverse-transcription PCR and agarose gel electrophoresis. The arrow indicates the correctly spliced PRSS1 band. The smaller faint band in the minigene samples indicated by the asterisk corresponds to an aberrant splice product in which nucleotide c.40 was spliced to c.114 resulting in the deletion of the mini-intron and an extra 74 nucleotides from exon 2.

    Article Snippet: The anti-PRSS1 sheep polyclonal antibody (catalog number AF3848, R&D Systems) was used at 1:5000 dilution.

    Techniques: Transfection, SDS Page, Staining, Activity Assay, Activation Assay, Western Blot, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Construct, Agarose Gel Electrophoresis